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LN-229 clones stably overexpressing HECTD1 as determined by Western blot (respective RNA expression levels of HECTD1 [ectopic and endogenous] are shown in Figure 6c) (a) were tested for adhesion-independent growth in soft agar (b). Quantification of colonies revealed strong reduction of colony formation in HECTD1 overexpressing cells (c) (data as mean +/-SD).
