Figure 2. Effect of FR5 on the proliferation of Bel7402 and HepG2 cells.

(A, B) Bel7402 and HepG2 cells were seeded into 96-well plates at a density of 5 × 10³ cells/well and then treated with FR5 at concentrations of 0, 20, 40, 80, 160 and 320 μg/ml for 24 and 48 h, and the cell viability rates were determined using MTS assay. (C, D) Bel7402 and HepG2 cells were treated with FR5 at concentrations of 0, 20, 40, 80, 160 and 320 μg/ml for 24 h with or without LY294002 pretreatment (25 μmol/L), and cell viability rates were examined and compared. The data are expressed in terms of percent of control cells as the means ± SD. The experiments were repeated at least three times. ^*p<0.05; ^**p<0.01 vs. control group.