Table 5.
Densitometric analysis of differentially (increase (↑) or decrease (↓)) expressed phosphotyrosine proteins (kDa) in U937 treated with various concentrations of AG18 (vs. 0 μM AG18) adherent on ligand-adsorbed TCPS at 24 h
| Ligands | AG18 concentration (μM)
|
|||
|---|---|---|---|---|
| 20 | 40 | 60 | 80 | |
| None (PBS) | NS | ↓~65, ~42, ~23 | ↓~42 | |
| ↑~200 | ↑~200,~23 | ↑~23 | ||
| Albumin | NS | ↓ ~52 | NS | |
| ↑~200,~160,~23 | ↑~23 | |||
| Fibronectin | ↓~200 (n),~160 (o) | ↓~23 | NS | |
| ↓~130 (p),~100 (q) | ↑~160 | ↑~42,~23 | ||
| G6 | ↓~85 (r),~70 (s), ~23 (t) | ↓~23 | ↑~23 | ↓ ~70,~23 |
| G3RGDG | ↓ ~200 (u),~130 (v), | ↓~23 | ↓~70 | |
| ↓ ~70 (w), ~42 (x) | ↑~200 | ↑~110,~70, ~42,~23 | ||
| G3PHSRNG | NS | ↓~52 | ↑~52 | |
| ↑~200,~160 | ↑~42,~23 | ↑~42 | ||
NS: no significant difference (p<0.05) when compared with 0 μM AG18 of respective surfaces. Letter in () indicates band designation in Fig. 5. Bold print indicates proteins that are differentially regulated when compared to cells treated with PMA only (Fig. 4). All values expressed in mean±s.d. and analyzed at p<0.05 (ANOVA of average values taken from two to four independent experiments (n = 2–4) with a culture condition of 50 ng/ml PMA, 5% FBS, seeding density of 1.7 × 105 cells/cm2 surface area) vs. 0 μMAG18 of respective surfaces.