Figure 2. The frequency of helper T cells (Th) within CD4⁺ population and TCRγδ within CD3⁺ cells is affected by FTY and DMF treatment.

The proportion of Th within CD4⁺ cells was determined by flow cytometric analysis of peripheral blood for identification of Th1 by CD119⁺ cells (A), Th2 by CD294⁺ cells (B), Th17 by CD196⁺ cells (C), Tfh by CXCR5⁺ cells (D), and Th22 by CCR10⁺ cells (E). (F) The frequency of TCRγδ within CD3⁺ T cells. The horizontal lines indicate the average mean of each group, and the bars are the SEM. p Values are indicated as insets; those reaching statistical significance are highlighted in red. DMF = dimethyl fumarate; FTY = fingolimod; NT = untreated; PBMC = peripheral blood mononuclear cell.