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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Clin Exp Allergy. 2017 Nov 22;48(1):29–38. doi: 10.1111/cea.13050

Figure 3. Eosinophils recruited to the lungs in response to fungal allergens do not depend on exogenous cytokine support for survival.

Figure 3

A. Survival of eosinophils isolated from lungs of IL5tg mice vs. eosinophils isolated from lungs of mice challenged with Aa (as in Fig. 1A) or sensitized and challenged with A. fumigatus (Af, see Methods); eosinophils were cultured in medium ex vivo without additional cytokines; n = 4 – 5 mice per time point, **p < 0.001, 2-way ANOVA. B. Survival of eosinophils isolated from the spleens of mice sensitized and challenged with A. fumigatus as in A. vs. spleens of IL5tg mice cultured ex vivo in medium without additional cytokines; n = 3 – 4 mice per time point, **p < 0.001, 2-way ANOVA. C. Survival of eosinophils isolated from lungs of IL5tg mice or D. isolated from the lungs of mice sensitized and challenged with Af, and cultured in medium with or without IL-13 (20 ng/mL); n = 5 mice per time point, *p < 0.05, **p < 0.001, 2-way ANOVA.