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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Clin Exp Allergy. 2017 Nov 22;48(1):29–38. doi: 10.1111/cea.13050

Figure 4. TNFα is detected in cultures of eosinophils from lungs of fungal-challenged mice, but this cytokine is not critical for ex vivo survival.

Figure 4

A. Immuno-reactive TNFα (but not IL5, IL-3, IL-13, eotaxin-1 or GM-CSF, see Suppl. Table 1) was detected in culture supernatants of eosinophils (106 cells/mL) from lungs of Af sensitized and challenged, but not IL5tg mice; n = 3 – 6 per point, **p < 0.01, *p < 0.05 vs. medium alone. Lower limit reported by manufacturer at 31.2 pg/mL; linear range was extended experimentally. B. Addition of anti-TNFα neutralizing antibody had no impact on sustained survival, n = 3 mice per point. C. Lung eosinophils isolated from TNFα gene-deleted mice were similarly capable of sustained survival, notably more robust on the C57BL/6 background; n = 5 – 10 mice per point.