Table 2. Cytokine contents from lung eosinophils.

Cytokine profiling of lysates of eosinophils isolated from lungs of mice challenged with A. alternata vs. eosinophils from IL5tg mice;

	Mean pixel density
Cytokine	A. alternata	IL5-tg	Ratio**
IL-2	889	1a	889
IL1-Ra	234737	149118	1.57
IL1-beta	2508	3061	0.82
IL1-alpha	4309	3650	1.18
IFN-gamma	2840	1248	2.28
Eotaxin-1	978	1a	978b
CCL1	1696	77	22.0
GM-CSF	2310	905	2.55
G-CSF	2076	530	3.92
C5-C5a	28487	4135	6.89
CXCL13	3307	1560	2.12
IL-27	1438	1105	1.30
IL-23	3395	25	136
IL-17	1898	1429	1.33
IL-16	2419	23265	0.10
IL-12p70	568	1a	568
IL-13	2177	1088	2.0
IL-7	2022	1294	1.56
IL-6	2011	1029	1.95
IL-5	756	1a	756
IL-4	1874	454	4.13
IL-3	2729	528	5.17
CXCL12	3014	2162	1.39
CCL5	3170	2132	1.49
CXCL2	18673	9720	1.92
CCL4	967	326	2.97
CCL3	10571	7359	1.44
CXCL9	2460	1674	1.45
CCL12	1096	102	10.7
CCL2	2177	549	3.97
M-CSF	6586	8433	0.78
KC	8053	1a	8050
I-TAC	2335	255	9.16
IP10	2389	410	5.83
TREM-1	9031	4860	1.86
TNF-alpha	4915	2895	1.70
TIMP-1	4722	2267	2.08
CCL17	448	1a	448

n = 5 mice (pooled lysates) per group as described in the Methods;

^abackground signal,

^bratio confirmed by DuoSet ELISA at 787 ± 210 pg eotaxin-1 per 10⁷ eosinophils from A. alternata challenged mice vs. 1.3 ± 1.3 pg eotaxin-1 per 1 × 10⁷ eosinophils from IL5tg mice, n = 3 – 4 mice per group,

^**p < 0.001.