Fig. 1. Generation of C. elegans model for transmission of HTT aggregates. (A) Scheme of the trans-cellular polyQ transmission model in C. elegans. (B) Transgenes used for generating the BiFC model in C. elegans. (C) Single-worm PCR analysis. Presence of transgenes were validated. V1Q25: 937-bp, V1Q97: 1050-bp; Q97V2: 761-bp, Q25V2: 557-bp. (D) Expression levels of polyQ proteins were measured by immunoblotting in each line. Three lines that have the similar expression levels were selected for experimental analysis (red numbers).