Fig. 2. Cell-to-Cell transmission of the polyQ protein aggregates in C. elegans. (A) Alteration of BiFC fluorescence with aging in the pharynx. The red arrowheads point to BiFC-positive inclusions in the pharynx. Scale bars: 200 µm. (B) Quantification of BiFC fluorescence with aging. Twenty worms for each line were used, ^*p<0.05. (C-E) BiFC-positive inclusions. Percentage of worms that have BiFC-positive inclusions at different ages (C, D). Worms with BiFC-positive inclusions were quantified separately in individual lines (E). Twenty worms for each line were used, n=3, ^***p<0.001.