Fig. 6. Regulation of cisplatin-induced apoptosis by ectopic expression of NAGA.

Cisplatin-resistant SK-OV-3 cells were transiently transfected with EGFP and NAGA expression constructs or with siNC and siNAGA. After 24 h of transfection, culture medium was replaced with fresh medium containing 30 µM cisplatin. Following 10 h of cisplatin treatment, cells were collected for western blot analysis to determine the Caspace-3 activation. All data is shown as the mean±SD of triplicate measurements. The statistical difference was determined by a t-test. siNC, non-targeting control siRNA; siNAGA, NAGA siRNA.