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. 2017 Nov 23;596(1):47–66. doi: 10.1113/JP275033

Figure 4. Effects of TGF‐β and ARHGEF1 siRNA on RhoA‐GTP content in hASMCs.

Figure 4

A, representative blots showing effects of acute BK treatment (1 μm, 30 s) with or without TGF‐β pre‐treatment (10 ng ml−1, 24 h) in hASMCs transfected with either ARHGEF1 siRNA or a scrambled siRNA control on RhoA‐GTP content, total RhoA or GAPDH as a loading control. B, data expressed as total/GAPDH show no overall effect of BK, TGF‐β or ARHGEF1 siRNA on total RhoA expression. C, data expressed as RhoA‐GTP/total show significant enhancement by BK (** P < 0.01 vs. control) but partial suppression by TGF‐β (## P < 0.01, vs. –TGF‐β) and ARHGEF1 siRNA ($ P < 0.05, $$ P < 0.01 vs. scrambled siRNA). n = 7 for all data. All comparisons by three‐way ANOVA.