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. 2017 Nov 23;596(1):47–66. doi: 10.1113/JP275033

Figure 6. Effects of TGF‐β and ARHGEF1 siRNA on expression and BK‐induced phosphorylation of MYPT‐1 in hASMCs.

Figure 6

A, representative blots showing effects of acute BK treatment (1 μm, 30 s) with or without TGF‐β pre‐treatment (10 ng ml−1, 24 h) in hASMCs transfected with either ARHGEF1 siRNA, or a scrambled siRNA control, on phospho‐MYPT‐1 (Thr‐696), total MYPT1 or GAPDH as a loading control. B, data expressed as total/GAPDH show enhancement of MYPT1 protein expression by TGF‐β pre‐treatment, but no effect of ARHGEF1 siRNA or BK, ## P < 0.01 vs. –TGF‐β. Data expressed as phospho/total (C) or phospho/GAPDH (D) show significant enhancement by both BK (* P < 0.05, ** P < 0.01 vs. control) and TGF‐β (# P < 0.05, ## P < 0.01 vs. –TGF‐β) and suppression by ARHGEF1 siRNA ($ P < 0.05, $$ P < 0.01 vs. scrambled siRNA). n = 9 for all data. All comparisons by three‐way ANOVA.