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. 2017 Nov 23;596(1):47–66. doi: 10.1113/JP275033

Figure 7. Effects of TGF‐β and ARHGEF1 siRNA on expression and BK‐induced phosphorylation of MLC20 in hASMCs.

Figure 7

A, representative blots showing effects of acute BK treatment (1 μm, 30 s) with or without TGF‐β pre‐treatment (10 ng ml−1, 24 h) in hASMCs transfected with either ARHGEF1 siRNA, or a scrambled siRNA control, on phospho‐MLC20 (Ser19), total MLC20 or GAPDH as a loading control. B, data expressed as total/GAPDH show enhancement of MLC20 protein expression by TGF‐β pre‐treatment (## P < 0.01 vs. –TGF‐β), and partial suppression of this response by ARHGEF1 siRNA ($$ P < 0.01 vs. scrambled siRNA). Data expressed as phospho/total (C) or phospho/GAPDH (D) show significant enhancement by both BK (* P < 0.05, ** P < 0.01 vs. control), effects of TGF‐β pre‐treatment (# P < 0.05, ## P < 0.01 vs. –TGF‐β) and suppression by ARHGEF1 siRNA ($ P < 0.05, $$ P < 0.01 vs. scrambled siRNA). n = 9 for all data. All comparisons by three‐way ANOVA.