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. 2017 Dec 1;596(1):83–103. doi: 10.1113/JP275089

Table 1.

Western analysis of intracellular signalling pathways

WB HS RE REM
Total Akt 0.32 ± 0.04 0.14 ± 0.02* 0.23 ± 0.03 0.20 ± 0.03*
Phospho‐Akt 0.52 ± 0.07 0.21 ± 0.06* 0.33 ± 0.05 0.36 ± 0.10
Total GSK3β 17.0 ± 1.6 11.2 ± 1.0 19.6 ± 2.6 22.2 ± 1.9
Phospho‐GSK3β 1.8 ± 0.2 1.2 ± 0.1 2.2 ± 0.3 2.6 ± 0.2
Total ERK1/2 28.8 ± 1.7 34.1 ± 1.9 31.1 ± 2.3 38.2 ± 1.5*
Phospho‐ERK1/2 1.9 ± 0.3 3.8 ± 1.1 4.0 ± 0.8 3.0 ± 0.9
Total FOXO3a 0.6 ± 0.1 0.5 ± 0.1 0.5 ± 0.1 0.6 ± 0.1
Phospho‐FOXO3a 0.5 ± 0.1 0.2 ± 0.0 0.3 ± 0.1 0.4 ± 0.0
Total rps6 3259 ± 441 2882 ± 347 4047 ± 379 4774 ± 539
Phospho‐rps6 970 ± 377 822 ± 308 3963 ± 677*, 3650 ± 854*,
HSP70 1.6 ± 0.3 0.7 ± 0.0 2.0 ± 0.3 2.3 ± 0.5

All values are in arbitrary density units. Values are means ± SEM. *Different from WB. Different from HS.