Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Shikonin-induced necroptosis causes a large release of glutamate from PanNET cells. (a) Appearance of cultures after 6 h incubation with control DMSO vehicle only (left) or 20 µM shikonin (right). Scale bars 100 µm. Extensive cell death and necrotic cell residue ‘ghosts’ are induced by shikonin. (b) Measurements of glutamate concentration in 1 ml of supernatant, after 90 min incubation of ≈300 000 cells in 20 µM shikonin (n = 16, ***p < 0.00002, Wilcoxon rank sum test).
