Figure 2.

One-dimensional ¹³C-spectrum for the seawater sample incubated over 2 days after the addition of ¹³C₆–glucose (Experiment 1). Patterns in the ¹³C–¹³C coupling (¹Jcc) for the major metabolites are shown as expanded panels. Italic numbers indicate the corresponding metabolites listed in Table 1. Greek letters in parentheses for amino acids denote the atom designation. For detailed analytical settings, see Table S1. In the panels showing the anomeric carbon of glucose (21 and 22), a spectrum determined for a standard compound of ¹³C₆–glucose is also shown (in brown), because the sample spectrum overlapped unknown signals (asterisks). Chemical structures were drawn by ACD/ChemSketch (ACD/Labs).