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. 2017 Dec 6;3(12):1322–1328. doi: 10.1021/acscentsci.7b00486

Figure 4.

Figure 4

Selenosulfide STEP inhibitor prodrugs. (a) Evaluation of STEP selenosulfide prodrug inhibitors 9 and 10 in the presence of a physiological intracellular concentration of GSH (1 mM). (b) Monitoring selenosulfide thiol exchange with GSH, amounts of 11 and 4 present determined by LCMS-UV. (c) Inhibition of STEP in rat cortical neurons treated with 9. Concentrations of 0.1, 1.0, or 10 μM of 9 for 1 h in HBS buffer and analyzed by Western blotting (*p < 0.05; **p < 0.01 one-way ANOVA, Dunnett’s post hoc). Data represent the phosphosignal normalized to the total protein signal and GAPDH + s.e.m. (n = 3–5 in each group).