Figure 5.

The microbicidal activity of biomimetic cationic NPs of PSS/DODAB and PSS/DODAB/Gr against S. aureus. Cell viability of S. aureus, in log (CFU/mL), as a function of DODAB concentration for different DODAB assemblies: (a) DODAB BF; (b) DODAB BF/Gr. where Gr concentration is 10% of [DODAB]; (c) PSS/DODAB; (d) PSS/DODAB/Gr. where Gr concentration is 10% of [DODAB]. Initial cell concentration was in the range (2–20) × 10⁷ CFU/mL. The ratio between DODAB and NP concentration was 10 µM DODAB per 2.4 × 10¹⁰ PSS particles/mL in (c) and (d). DODAB and Gr concentration varied with the particle number density in (c) and (d). After 1 h of interaction between cells and assemblies, dilution and plating of the mixtures allowed CFU counting.