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. 2017 Dec 29;10:626. doi: 10.1186/s13071-017-2526-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Clonorchis sinensis-specific IgG, IgG1, Ig2a, IgG2b and IgG2c antibody responses in rats immunized with VLPs. Clonorchis sinensis-specific IgG (a), IgG1 (b), IgG2a (c), IgG2b (d) and IgG2c (e) antibody responses were determined in the sera at weeks 1 and 4 from primary (Imm 1st-w1) and secondary immunization (Imm 2nd-w4). Significantly higher levels of IgG, IgG1, IgG2a and IgG2c antibody responses were observed after boost compared to those after prime (*P < 0.05, **P < 0.01)