Table 6.
Effect of Doxorubicin (DOX) and different treatments of AHE on cardiac tissue protein, H2O2, nitrite content and lipid peroxidation
| Group | Protein (μg/mg Tissue) | H2O2 (nM/min/mg Tissue) | Nitrite (content μM/ml) | TBARS (nM/min/mg protein) |
|---|---|---|---|---|
| Control | 1.638 ± 0.033b | 1.932 ± 0.015b | 42.59 ± 0.552b | 2.874 ± 0.180b |
| DOX | 1.122 ± 0.0323a | 5.854 ± 0.011a | 78.12 ± 0.499a | 7.575 ± 0.573a |
| AHE alone | 1.614 ± 0.015b | 1.911 ± 0.049b | 41.72 ± 0.650b | 2.859 ± 0.086b |
| DOX + AHE (200) | 1.392 ± 0.036a,b | 3.950 ± 0.003a,b,d | 66.47 ± 1.456a,b,d | 6.159 ± 0.091a,b*,d |
| DOX + AHE (400) | 1.490 ± 0.018a*,b | 2.719 ± 0.006a,b,c | 49.94 ± 0.770a,b,c | 3.258 ± 0.167b,c |
| DOX + Sily | 1.509 ± 0.027b | 2.645 ± 0.004a,b | 50.60 ± 0.322a,b | 3.233 ± 0.151b |
Values expressed as mean ± SEM
aSignificance at p < 0.0001 Vs. control group
bSignificance at p < 0.0001 Vs. Doxorubicin (DOX) group
cSignificance at p < 0.0001 of DOX + AHE 400 mg/kg group Vs. DOX + AHE 200 mg/kg group
dSignificance at p < 0.0001 of AHE co-treatment groups Vs DOX + Sily group
*Significant difference at p < 0.05 and p < 0.001 respectively. Non-significant difference (p > 0.05) was recorded between control and AHE alone treated group in all parameters. (One way ANOVA followed by Tukey’s multiple comparison tests). Sily-Silymarin