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. 2017 Dec 29;22:33. doi: 10.1186/s11658-017-0065-x

Fig. 2.

Fig. 2

Enforced expression of TWIST1 upregulates OCT4 mRNA expression in KYSE30 cells. aTWIST1 is overexpressed nine-fold in GFP-hTWIST1 compared to the control. This caused a statistically significant eight-fold increase in OCT4 mRNA expression. b – Phase contrast and fluorescent microscope images of GFP-hTWIST1 and control cells. The phase contrast images show the cells after 24 h and the fluorescent images are after 48 h