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. 2017 Dec 29;18:996. doi: 10.1186/s12864-017-4373-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Relative CBP mRNA levels in Tribolium determined by qRT-PCR. Samples were collected at 24 h intervals during penultimate larvae, final instar larvae and pupal stages. Total RNA was extracted from pools of two larvae/pupae for each time period and converted to cDNA. The cDNA and gene-specific primers were used to quantify CBP mRNA levels using Ribosomal Protein 49 (Rp49) mRNA levels for normalization. The CBP mRNA levels in newly molted penultimate larvae were set as 1. Mean + SE of four replications were shown (Letters represent significance at 95% CI)