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. Author manuscript; available in PMC: 2017 Dec 29.
Published in final edited form as: Methods Enzymol. 2017 Jun 9;591:159–186. doi: 10.1016/bs.mie.2017.03.013

Fig 1. Reporter constructs.

Fig 1

A. TEMrev-GFP reporter. Main features: Lac promoter: 143-172; LacZ fusion: 217-288; Cycle 3 GFP: 289-1005; Kan. Res. 1219-2004; β-lactamase: 2291-3151 (2492-2494 S70X reversion reporter); ColE1-like (pMB1) plasmid origin of replication 3299-4091. In the TEMrev-GFPrev variant, the Q183R mutant codon is at positions 835-837. The mutant codon is CGA (R), which requires a G→A transition to revert to CCA (Q). B sfGFP-TEM reporter. pMB1 ori 3999-479; B sfGFP lac promoter 143-172; lacZ fusion 223-259, sfGDP: 201-1014; 12 aa serine/glycine-rich linker 1015-1050; lactamase 1051-1911; the K126stop codon is at positions 579-81; M13 ori: 1953-2462; Kan resistance (opposite orientation) 3400-2575; lactamase fragment 3553-3851.