Table 2.
Pol I | Temperature | Total colonies screened (×104) | Total number of transformations | Average transformation efficiency | Number of fluorescent colonies | Frequency of GFP reversion (×106) | S70P reversion frequency (×106) |
---|---|---|---|---|---|---|---|
WT | 30 °C | 60.4 | 6 | 10.1 | 0 | <1.7 | N/A |
WT | 37 °C | 18.1 | 6 | 3.0 | 3 | 16.5 | 0.004 |
LF | 30 °C | 13.4 | 8 | 1.6 | 5 | 37.2 | N/A |
LF | 37 °C | 5.1 | 8 | 0.6 | 7 | 137.0 | 180.9 |