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. Author manuscript; available in PMC: 2017 Dec 29.
Published in final edited form as: Methods Enzymol. 2017 Jun 9;591:159–186. doi: 10.1016/bs.mie.2017.03.013

Table 2.

GFP reversion frequencies, compared to those of S70P

Pol I Temperature Total colonies screened (×104) Total number of transformations Average transformation efficiency Number of fluorescent colonies Frequency of GFP reversion (×106) S70P reversion frequency (×106)
WT 30 °C 60.4 6 10.1 0 <1.7 N/A
WT 37 °C 18.1 6 3.0 3 16.5 0.004
LF 30 °C 13.4 8 1.6 5 37.2 N/A
LF 37 °C 5.1 8 0.6 7 137.0 180.9