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. Author manuscript; available in PMC: 2017 Dec 29.
Published in final edited form as: Cell Rep. 2017 Dec 26;21(13):3691–3699. doi: 10.1016/j.celrep.2017.12.004

Figure 3. A truncated RLIM340 protein is expressed in Rlim340f ESCs.

Figure 3

A) Western blot on undifferentiated ESCs was consecutively hybridized with RLIM-M, β-actin and REX1 antibodies. Note the presence of RLIM340 in Rnf12KO (Rlim340f) ESCs. Moreover, REX1 levels are elevated in Rlim340f ESCs but not in RlimKOp ESCs. B) Forced expression of Myc-tagged RLIM340 in RlimKOp ESCs leads to nuclear accumulation of endogenous REX1. C) RLIM340 traps REX1 in the nucleus. ICC on undifferentiated ESCs using REX1 antibodies. Note mostly nuclear localization of REX1 in Rlim340f but not in Rlim53f and Rlim0f ESCs. D) Presence of RLIM340 inhibits XCI efficiency. Summary of Xist clouds from three independent experiments in Rlim340f, Rlim53f and Rlim0f ESCs EB-differentiated for 6d. From each experiment Xist clouds in ESCs of 10 EBs were evaluated with >100 cells counted per EB. Error bars indicate SEM. See also Figure S2.