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. 2017 Nov 19;591(23):3850–3860. doi: 10.1002/1873-3468.12898

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© 2017 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Reliability of the extended beta distribution fit. (A) I _app of Kcv_NTS at 1 m KCl as obtained with the 1‐kHz filter (black circles) or from filtering the 10‐kHz data from Meca‐4 chips to ca. 1 kHz by a moving average filter (blue circles). I _OF as determined by the extended beta distribution fit with 1‐kHz filter [Eqn (1)], open black circles, n = 3) and I _OF directly measured with the 10‐kHz filter (open red circles n = 4). (B) Representative current traces of Kcv_NTS in 1 m KCl filtered at 10 kHz. Closed channel and I _OF are labeled as C and OF, respectively. (C) Current I _app measured at 1 kHz for Kcv_NTS at 0.1 m (black) and 1.5 m KCl (blue) compared with I _app (gray and orange) as calculated from averaging over O‐M and O‐F gating [Eqn (2)]. Mean and standard deviation of 6 and 3 experiments at 0.1 and 1.5 m, respectively.