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. Author manuscript; available in PMC: 2018 Feb 11.
Published in final edited form as: Science. 2017 Aug 11;357(6351):609–612. doi: 10.1126/science.aaj1849

Fig. 3. Disturbance of layer interfaces affects chromatin structure, gene transcription and growth of T. kodakarensis.

Fig. 3

A) Growth curves of T. kodakarensis TS600 and derivative strains with the HTkA G17 substitutions indicated, in medium containing S° and following dilution into a medium lacking S° (pyruvate). Error bars show the SD from three independent experiments, each run with triplicate cultures. B) Quantitative RT-PCR of transcripts of three genes in the hydrogenase operon (TK2080-TK2081-TK2088) present in T. kodakarensis cells containing HTkA or HTkA G17L grown in the presence or absence of S°. Transcripts of TK0895, TK1431 and TK1311 were quantified as constitutively expressed reference genes. Shown is the fold change of the hydrogenase transcripts in cells following dilution into a medium lacking S° (pyruvate). C) DNA fragments generated by MNase digestion of chromatin isolated from T. kodakarensis TS600 and derivative TS620 (G17D) and TS621 (G17L). DNA stripped from histones prior to MNase digestion is shown as a control (TS600). Size standards are in lanes M.