Table 1. List of primers and probes.
| Gene | Forward primer
(5’ – 3‘) |
Reverse primer
(5’ – 3‘) |
Probe
(5’ – 3‘) |
Reference or accession number |
|---|---|---|---|---|
| IPNV SA | TCTCCCGGGCAGTTCAAGT | CGGTTTCACGATGGGTTGTT | CCAGAACCAGGTGACGAGTATGAGGACTACAT | 20 |
| ef1α | ACCCTCCTCTTGGTCGTTTC | TGATGACACCAACAGCAACA | GCTGTGCGTGACATGAGGCA | 44 |
| tlr3 | ACTCGGTGGTGCTGGTCTTC | GAGGAGGCAATTTGGACGAA | CAAGTTGTCCCGCTGTCTGCTCCTG | NM_001124578.1 |
| irf7 | CCCAGGGTTCAGCTCCACTA | GGTCTGGCAACCCGTCAGT | TCGAGCCAAACACCAGCCCCT | AJ829673 |
| ifn1 | ACCAGATGGGAGGAGATATCACA | GTCCTCAAACTCAGCATCATCTATGT | AATGCCCCAGTCCTTTTCCCAAATC | AM489418.1 |
| mx1–3 | TGAAGCCCAGGATGAAATGG | TGGCAGGTCGATGAGTGTGA | ACCTCATCAGCCTAGAGATTGGCTCCCC | 45 |
| pkr | GACACCGCGTACCGATGTG | GGACGAACTGCTGCCTGAAT | CACCACCTCTGAGAGCGACACCACTTC | NM_001145891.1 |
| il8 | AGAGACACTGAGATCATTGCCAC | CCCTCTTCATTTGTTGTTGGC | 46 | |
| ifnγ | CAAACTGAAAGTCCACTATAAGATCTCCA | TCCTGAATTTTCCCCTTGACATATTT | 47 | |
| tnfα | AGCATGGAAGACCGTCAACGAT | ACCCTCTAAATGGATGGCTGCTT | 48 |