Table 2. Rt-qPCR virus titration.
Ct value ± standard deviation from standard line points (10 8 to 10 2 dilutions) and supernatans from IPNV-exposed RBCs at MOI 0.5, at 3 and 6 dpe. (n=7 individuals).
| Sample | Ct value ± SD |
|---|---|
| 10 8 TCID 50 | 25,885 ± 0,052 |
| 10 7 TCID 50 | 29,856 ± 0,117 |
| 10 6 TCID 50 | 33,165 ± 0,168 |
| 10 5 TCID 50 | 36,057 ± 0,11 |
| 10 4 TCID 50 | 39,126 ± 0.873 |
| 10 3 TCID 50 | Undetected |
| 10 2 TCID 50 | Undetected |
| RBCs #1 3 dpe | Undetected |
| RBCs #1 6 dpe | Undetected |
| RBCs #2 3 dpe | Undetected |
| RBCs #2 6 dpe | Undetected |
| RBCs #3 3 dpe | Undetected |
| RBCs #3 6 dpe | Undetected |
| RBCs #4 3 dpe | Undetected |
| RBCs #4 6 dpe | Undetected |
| RBCs #5 3 dpe | Undetected |
| RBCs #5 6 dpe | Undetected |
| RBCs #6 3 dpe | Undetected |
| RBCs #6 6 dpe | Undetected |
| RBCs #7 3 dpe | Undetected |
| RBCs #7 6 dpe | Undetected |
| NTC | Undetected |