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. 2017 Dec 29;12(12):e0190356. doi: 10.1371/journal.pone.0190356

Fig 1. Mild heat exposure accelerates neural stem/progenitor cell proliferation rate under proliferation culture conditions.

Fig 1

(A) NSCs/NPCs were cultured in proliferation media (PM) at either 37.0°C or 38.5°C for 4 days. (B) On day 4 of culture in PM, viability was measured by MTS cell viability assay. Data are expressed as percentages of the control temperature group (37.0°C). Results are mean ± SEM of five independent experiments. *P < 0.05 vs. control temperature. (C) On day 4, neurospheres generated at control temperature and under mild heat exposure were dissociated separately by trituration and the total number of cells counted by trypan blue exclusion. Values are mean ± SEM of five independent experiments. *P < 0.05 vs. control temperature. (D) Representative images of neurospheres formed at 37.0°C or 38.5°C on day 4 of proliferation. Bar indicates 250 μm. (E) Quantitative analysis of neurosphere size distribution arbitrarily divided into four classes according to diameter. Data are expressed as percentages of the total number of neurospheres. Results are mean ± SEM of four independent experiments. *P < 0.05 vs. control temperature.