Video 4. Calcium signals in adoptively transferred Cd4-Salsa6f T cells.

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DOI: 10.7554/eLife.27827.015

Red signal from tdTomato expression in cytosol facilitates identification and tracking of cells; green GCaMP6f signal detects elevation of Ca²⁺. Autofluorescent structures appear as stationary yellow bodies. Movie is paused at frame 323, zoomed in to emphasize two examples of Ca²⁺ transients and an autofluorescent body. Images were acquired at 5 s interval. Major tick marks at 20 µm. Playback speed = 50 frames/s, time shown in hr:min:s. Video corresponds to Figure 7A.