Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers |
|---|---|---|---|
| Strain, strain background (Mus musculus) | B6.Cg-Foxp3tm1Mal/J | Wang et al. (2008) (PMID: 18209052) | IMSR Cat# JAX:018628, RRID:IMSR_JAX:018628 |
| Strain, strain background (Mus musculus) | B6.Cg-Tg(TcraTcrb)425Cbn/J | Barnden et al., 1998(PMID: 9553774) | IMSR Cat# JAX:004194, RRID:IMSR_JAX:004194 |
| Antibody | Alexa Fluor 700-conjugated anti CD45.2 (104) | BD Biosciences | Cat# 560693 |
| Antibody | Allophycocyanin (APC)-conjugated anti-CD25 (PC61) | BD Biosciences | Cat# 561048 |
| Antibody | Allophycocyanin (APC)-conjugated anti-CD44 (IM7) | BD Biosciences | Cat# 561862 |
| Antibody | Brilliant Violet (BV) 421-conjugated anti Ly-6C (AL-21) | BD Biosciences | Cat# 562727 |
| Antibody | BV 510-conjugated anti-CD4 (RM4-5) | BD Biosciences | Cat# 563106 |
| Antibody | BV 786-conjugated anti-CD25 (PC61) | BD Biosciences | Cat# 564023 |
| Antibody | Phycoerythrin (PE)-conjugated anti-CD25 (PC61) | BD Biosciences | Cat# 561065 |
| Antibody | Phycoerythrin (PE)-conjugated anti-CD69 (H1.2F3) | BD Biosciences | Cat# 553237 |
| Antibody | Phycoerythrin (PE)-conjugated anti-Izumo1r (TH6) | BD Biosciences | Cat# 560320 |
| Antibody | Phycoerythrin (PE)-conjugated anti-TCRgd (GL3) | BD Biosciences | Cat# 553178 |
| Antibody | Phycoerythrin (PE)-conjugated anti-Vb5.1/5.2 (MR9-4) | BD Biosciences | Cat# 553190 |
| Antibody | PE-Cy7-conjugated anti-CD44 (IM7) | BD Biosciences | Cat# 560569 |
| Antibody | PE-Cy7-conjugated anti-CD45.1 (A20) | BD Biosciences | Cat# 560578 |
| Antibody | Biotinylated anti-CD5 (53–7,3) | BD Biosciences | Cat# 553019 |
| Antibody | Biotinylated anti-CD62L (MEL14) | BD Biosciences | Cat# 553149 |
| Antibody | Biotinylated anti-Ly-6C (AL-21) | BD Biosciences | Cat# 557359 |
| Antibody | Biotinylated anti-Sca1 (E13-161.7) | BD Biosciences | Cat# 553334 |
| Antibody | Alexa Fluor 647-conjugated anti-IL18ra (BG/IL18ra) | BioLegend | Cat# 132903 |
| Antibody | APC-conjugated streptavidin | BioLegend | Cat# 405207 |
| Antibody | BV 421-conjugated anti-Ly-6C (HK1.4) | BioLegend | Cat# 128032 |
| Antibody | PE-conjugated anti-Ly-6C (HK1.4) | BioLegend | Cat# 128008 |
| Antibody | Alexa 448-conjugated anti-NFAT2 (7A6) | BioLegend | Cat# 649603 |
| Antibody | Alexa 448-conjugated anti-NFAT1 (D43B1) | Cell Signaling | Cat# 14324 |
| Antibody | PE-conjugated anti-CD200 (OX-90) | eBioscience | Cat# 12-5200-82 |
| Antibody | PE-conjugated anti-Ikzf3 (8B2) | eBioscience | Cat# 12-5789-80 |
| Antibody | PE-conjugated anti-Nur77 (12.14) | eBioscience | Cat# 12-5965-82 |
| Antibody | PerCP-Cy5.5-conjugated anti-TCRb (H57-597) | eBioscience | Cat# 45-5961-82 |
| Antibody | Biotinylated anti-CD73 (eBioTY/11.8) | eBioscience | Cat# 14-0731-82 |
| Antibody | APC-conjugated anti-Foxp3 (FJK-165) | eBioscience | Cat# 12-5773-82 |
| Antibody | PE-conjugated anti-Foxp3 (FJK-165) | eBioscience | Cat# 17-5773-82 |
| Antibody | Pacific Blue-conjugated streptavidin | Invitrogen | Cat# S11222 |
| Antibody | APC-Vio770-conjugated anti-CD8a (53–6.7) | Miltenyi Biotec | Cat# 130-102-305 |
| Antibody | PE-conjugated anti-CD122 (TM-b1) | Miltenyi Biotec | Cat# 130-102-569 |
| Chemical compound, drug | Phorbol 12-myristate 13-acetate (PMA) | Calbiochem | CAS 16561-29-8 |
| Chemical compound, drug | FK506 (tacrolimus) | Sigma Aldrich | CAS 109581-93-3 |
| Chemical compound, drug | CellTrace Violet | Invitrogen | Cat# C34557 |
| Chemical compound, drug | CellTrace Far Red | Invitrogen | Cat# C34564 |
| Chemical compound, drug | Recombinant Mouse IL-7 | R and D Systems | Cat# 407 ML-025 |
| Chemical compound, drug | Thapsigargin | Calbiochem | CAS 67526-95-8 |
| Chemical compound, drug | TGFβ1 | Invitrogen | Cat# PHG9204 |
| Chemical compound, drug | DRAQ5 | Cell Signaling | Cat# 4084 |
| Chemical compound, drug | Indo-1, AM | Invitrogen | Cat# I1223 |
| Software, algorithm | Illustrator CS5 | Adobe Systems Inc. | http://www.graphpad.com |
| Software, algorithm | GeneChip Scanner 3000 7G | Affymetrix | N/A |
| Software, algorithm | Expression Console | Affymetrix | https://imagej.nih.gov/ij/ |
| Software, algorithm | DIVA8.0.1 | BD Biosciences | N/A |
| Software, algorithm | R | Bioconductor | N/A |
| Software, algorithm | Prism 7 | GraphPad | N/A |
| Software, algorithm | ImageJ | NIH | https://www.bioconductor.org/ |
| Software, algorithm | Partek Genomics Suite | Partek | N/A |
| Deposited data | GSE14308 | Wei et al. (2009), PMID: 19144320 | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE14308 |
| Deposited data | GSE42276 | Wakamatsu et al. (2013), PMID: 23277554 | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE42276 |
| Deposited data | GSE67464 | Bevington et al., 2016, PMID: 26796577 | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE67464 |
| Deposited data | GSE70154 | Richards et al. (2015), PMID: 26195815 | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70154 |
| Deposited data | GSE62532 | Vahl et al. (2014), PMID: 25464853 | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE62532 |
| Deposited data | GSE97477 | This paper | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE97477 |