Video 3. 4D reconstruction of cellular calcium transients.

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DOI: 10.7554/eLife.28307.015

(1) 4D reconstruction of calcium activation from the synchronized planar movies recorded in the heart of a living Tg(myl7:GCaMP5G) zebrafish embryo at 52 hpf. The number of slices used in the reconstruction is indicated by n. Raw GCaMP5G signal is shown in orange. Time is indicated in ms. (2) Cell detection: The raw fluorescence signal of myl7:H2A-mCherry is overlaid with the centroids of detected nuclei. (3) Calcium mapping: The raw myl7:GCaMP5G signal is shown. The dots indicate the extracted cellular positions. (4) Cell-specific transients: The normalized GCaMP5G transients are shown for two sample cells (one atrial and one ventricular cell).