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. 2017 Dec 12;6:e30115. doi: 10.7554/eLife.30115

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© 2017, Ahn et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 2. — (A) Drawing of the fifth tarsal segment of the prothoracic leg that is used for immunofluorescence experiments (B–F). Tarsal taste sensilla are indicated. (B and C) IR25a and IR76b are expressed in sweet GRNs. Immunostaining with anti-GFP (green) and anti-mCD8 (B; magenta) or anti-HA (C; magenta) antibodies on whole-mount preparations of the fifth tarsal segment of the prothoracic leg from flies of the genotypes: IR25a-GAL4/UAS-mCD8:RFP; Gr64f^LexA lexAop-rCD2:GFP (B) or IR76b-QF UAS-mCD8:GFP/Gr64f-GAL4;QUAS-mtd-Tomato-3xHA/+ (C). Arrows refer to a GRN of a sensillum expressing both Gr64f and the indicated IR genes. (D and E) IR25a and IR76b are expressed in bitter GRNs. Immunostaining with anti-GFP (green) and anti-IR25a (D; magenta) or anti-HA (E; magenta) antibodies on whole-mount preparations of the fifth tarsal segment of the prothoracic leg from flies of the genotypes: Gr33a^GAL4/UAS-mCD8:GFP (D) or IR76b-QF UAS-mCD8:GFP/Gr33a^GAL4;QUAS-mtd-Tomato-3xHA/+ (E). Arrows refer to a GRN of a sensillum expressing both Gr33a and the indicated IR genes. (F) IR25a and IR76b are largely co-expressed in tarsal GRNs. Immunostaining with anti-GFP (green) and anti-HA (magenta) antibodies on whole-mount preparations of the fifth tarsal segment of the prothoracic leg from flies of the genotypes: IR76b-QF UAS-mCD8:GFP/IR25a-GAL4;QUAS-mtd-Tomato-3xHA/+. Arrows refer to GRNs expressing both IR25a and IR76b genes. Numbers indicate the average count of IR or Gr expressing GRNs/sensillum. Due to close proximity, neurons could not always be associated with either the 5s and 5v sensillum, and the cell count was therefore pooled.

Figure 2—figure supplement 1. — (A) Drawing of the fifth tarsal segment of the prothoracic leg that is used for immunofluorescence experiments (B–F). Tarsal taste sensilla are indicated. (B and C) IR25a and IR76b are expressed in sweet GRNs. Immunostaining with anti-GFP (green) and anti-mCD8 (B; magenta) or anti-HA (C; magenta) antibodies on whole-mount preparations of the fifth tarsal segment of the prothoracic leg from flies of the genotypes: IR25a-GAL4/UAS-mCD8:RFP; Gr64f^LexA lexAop-rCD2:GFP (B) or IR76b-QF UAS-mCD8:GFP/Gr64f-GAL4;QUAS-mtd-Tomato-3xHA/+ (C). Arrows refer to a GRN of a sensillum expressing both Gr64f and the indicated IR genes. (D and E) IR25a and IR76b are expressed in bitter GRNs. Immunostaining with anti-GFP (green) and anti-IR25a (D; magenta) or anti-HA (E; magenta) antibodies on whole-mount preparations of the fifth tarsal segment of the prothoracic leg from flies of the genotypes: Gr33a^GAL4/UAS-mCD8:GFP (D) or IR76b-QF UAS-mCD8:GFP/Gr33a^GAL4;QUAS-mtd-Tomato-3xHA/+ (E). Arrows refer to a GRN of a sensillum expressing both Gr33a and the indicated IR genes. (F) IR25a and IR76b are largely co-expressed in tarsal GRNs. Immunostaining with anti-GFP (green) and anti-HA (magenta) antibodies on whole-mount preparations of the fifth tarsal segment of the prothoracic leg from flies of the genotypes: IR76b-QF UAS-mCD8:GFP/IR25a-GAL4;QUAS-mtd-Tomato-3xHA/+. Arrows refer to GRNs expressing both IR25a and IR76b genes. Numbers indicate the average count of IR or Gr expressing GRNs/sensillum. Due to close proximity, neurons could not always be associated with either the 5s and 5v sensillum, and the cell count was therefore pooled.