Key resources table.
| Reagent type | Designation | Source or reference | Identifiers |
|---|---|---|---|
| Antibodies | |||
| Chicken polyclonal anti-GFP | Anti-GFP | Thermo Fisher Scientific | PA1-86341; RRID: AB_931091 |
| Rat monoclonal anti-mCD8 | Anti-mCD8 | Thermo Fisher Scientific | MCD0800; RRID: AB_10392843 |
| Rabbit polyclonal anti-IR25a | Anti-IR25a | Benton et al. (2009) | N/A |
| Mouse monoclonal anti-HA | Anti-HA | Covance Research Product Inc. | MMS-101P; RRID: AB_2314672 |
| Alexa 488 conjugated goat anti-chicken | Green | Thermo Fisher Scientific | A11039; RRID: AB_2534096 |
| Cy3-conjugated goat anti-rat | Magenta | Jackson Immunoresearch Laboratories Inc. | 112-165-072; RRID: AB_2338248 |
| Cy3-conjugated goat anti-rabbit | Magenta | Jackson Immunoresearch Laboratories Inc. | 111-166-003; RRID: AB_2338000 |
| Cy3-conjugated goat anti-mouse | Magenta | Jackson Immunoresearch Laboratories Inc. | 115-166-072; RRID: AB_2338706 |
| Chemical compounds | |||
| Hexanoic acid | Hexanoic acid | Sigma-Aldrich | H12137 |
| Octanoic acid | Octanoic acid | Sigma-Aldrich | O3907 |
| Linoleic acid | Linoleic acid | Sigma-Aldrich | L1376 |
| Denatonium benzoate | Denatonium | Sigma-Aldrich | D5765 |
| Sucrose | Sucrose | Amresco | M1117 |
| Experimental Models: Organisms/Strains | |||
| D. melanogaster: w1118 | Wild-type control | Bloomington Drosophila Stock Center | BDSC: 3605; FlyBase: FBst0003605 |
| D. melanogaster: w*; P{Gr64f-GAL4.9.7}5/CyO; MKRS/TM2 | Gr64f-GAL4 | Dahanukar et al. (2007) | Flybase: FBst0057669 |
| D. melanogaster: w*; TI{LexA::VP16}Gr64fLexA | Gr64fLexA | Fujii et al. (2015) | Flybase: FBti0168176 |
| D. melanogaster: w*; TI{GAL4}Gr33aGAL4 | Gr33aGAL4 | Moon et al. (2009) | Flybase: FBst0031425 |
| D. melanogaster: w*; P{UAS-TeTxLC.tnt}G2 | UAS-TNT | Sweeney et al. (1995) | Flybase: FBst0028838 |
| D. melanogaster: w*; P{lexAop-rCD2-GFP} | lexAop-rCD2:GFP | Lai and Lee (2006) | Flybase: FBst0066687 |
| D. melanogaster: w*;P{10XUAS-IVS-mCD8::RFP}attP40 | UAS-mCD8:RFP | Bloomington Drosophila Stock Center | BDSC: 32219; Flybase: FBti0131967 |
| D. melanogaster: w1118; PBac{20XUAS-IVS-GCaMP6m}VK00005 | UAS-GCaMP6m | Bloomington Drosophila Stock Center | BDSC: 42750; Flybase: FBst0042750 |
| D. melanogaster: y1 w*; P{UAS-mCD8::GFP.L}LL5,P{UAS-mCD8::GFP.L}2 | UAS-mCD8:GFP | Bloomington Drosophila Stock Center | BDSC: 5137; Flybase: FBst0005137 |
| D. melanogaster: y1 w1118; P{QUAS-mtdTomato-3xHA}26 | QUAS-mtd-Tomato-3xHA | Bloomington Drosophila Stock Center | BDSC: 30005; Flybase: FBst0030005 |
| D. melanogaster: y1 sc* v1; P{TRiP.HMC03664}attP40 | UAS-IR94h-RNAi | Bloomington Drosophila Stock Center | BDSC: 53675; Flybase: FBst0053675 |
| D. melanogaster: w1118; Mi{ET1}IR52cMB04402 | Mi{ET1}IR52cMB04402 | Bloomington Drosophila Stock Center | BDSC: 24580; Flybase: FBst0024580 |
| D. melanogaster: w1118; Mi{ET1}IR56bMB09950 | Mi{ET1}IR56bMB09950 | Bloomington Drosophila Stock Center | BDSC: 27818; Flybase: FBst0027818 |
| D. melanogaster: y1 w*;Mi{MIC}IR62aMI00895 lml1MI00895/TM3, Sb1, Ser1 | Mi{Mic}IR62aMI00895 | Bloomington Drosophila Stock Center | BDSC: 32713; Flybase: FBst0032713 |
| D. melanogaster: w1118;P{UAS-norpA.WT}2 | UAS-norpA | Bloomington Drosophila Stock Center | BDSC: 35529; Flybase: FBst0035529 |
| D. melanogaster: w*; TI{TI}IR25a1/CyO | IR25a1/CyO | Benton et al. (2009) | Flybase: FBst0041737 |
| D. melanogaster: w*; P{IR25a-GAL4.A}236.1; TM2/TM6B, Tb1 | IR25a-GAL4 | Abuin et al. (2011) | Flybase: FBst0041728 |
| D. melanogaster: w*; M{UAS-IR25a.attB} | UAS-IR25a | Abuin et al. (2011) | Flybase: FBal0249355 |
| D. melanogaster: {KK104276}VIE-260B | UAS-IR11a-RNAi | Vienna Drosophila Resource Center | VDRC ID: 100422; Flybase: FBgn0030385 |
| D. melanogaster: w1118; P{GD773}v2472 | UAS-IR21a-RNAi | Vienna Drosophila Resource Center | VDRC ID: 2472; Flybase: FBgn0031209 |
| D. melanogaster: w1118; P{GD2094}v4704 | UAS-IR56b-RNAi | Vienna Drosophila Resource Center | VDRC ID: 4704; Flybase: FBgn0034456 |
| D. melanogaster: w1118; P{GD2096}v6112 | UAS-IR56d-RNAi | Vienna Drosophila Resource Center | VDRC ID: 6112; Flybase: FBgn0034458 |
| D. melanogaster: w*; IR76b1 | IR76b1/IR76b1 | Zhang et al. (2013) | Flybase: FBst0051309 |
| D. melanogaster: w*; IR76b2 | IR76b2/IR76b2 | Zhang et al. (2013) | Flybase: FBst0051310 |
| D. melanogaster: w*; P{IR76b-GAL4.1.5}2 | IR76b-GAL4, | Zhang et al. (2013) | Flybase: FBst0051311 |
| D. melanogaster: w*; P{UAS-IR76b.Z}2/CyO; TM2/TM6B, Tb1 | UAS-IR76b | Zhang et al. (2013) | Flybase: FBst0052610 |
| D. melanogaster: w*; P{IR76b-QF.1.5} | IR76b-QF | Zhang et al. (2013) | Flybase: FBtp0085487 |
| D. melanogaster: w*; P{UAS-Hsap\KCNJ2.EGFP}1 | UAS-Kir2.1-GFP | Baines et al. (2001); Paradis et al. (2001) | Flybase: FBst0006596 |
| D. melanogaster: w*; norpA36(P24) | norpAP24 | Masek and Keene (2013) | Flybase: FBst0009048 |
| D. melanogaster: w*; P{IR56d-GAL4.K}7–2/CyO; P{UAS-mCD8::GFP.L}LL6 | IR56d-GAL4 | Koh et al. (2014) | Flybase: FBst0060708 |
| D. melanogaster: w*; Gr66a-LexA/CyO; TM2/TM6B | Gr66a-LexA | Thistle et al. (2012) | Flybase: FBal0277069 |
| Software and Algorithms | |||
| NIS-Elements | N/A | Nikon | N/A |
| Prism software | Prism software | GraphPad Software 5.0 Inc | N/A |
| Other | |||
| Nikon Eclipse Ti inverted microscope | Nikon Eclipse Ti inverted microscope | Nikon | N/A |
| Nikon A1 confocal microscope | Nikon A1R confocal microscope system | Nikon | N/A |
| 35 mM Glass bottom dish | Glass bottom culture dish | MatTek Corporation | P35G-0–10 C |