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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Glia. 2017 Aug 29;65(12):1976–1989. doi: 10.1002/glia.23208

FIGURE 4.

FIGURE 4

TLQP-21-evoked Ca2+ transients in dorsal horn are C3aR1-dependent. (a) The total number of profiles responsive to 1 μM TLQP-21 and (b) peak TLQP-21-evoked Ca2+ signals decrease in the presence of 1 μM SB290157 (n = 4 mice/group). (c) Representative ratiometric traces of [Ca2+]i in lamina II dorsal horn from a mouse spinal cord slice exposed to 1 μM R21A (90 s), 1 μM TLQP-21 (90 s) and 1 mM glutamate (10 s). Note that the TLQP-21 responsive profile does not respond to glutamate. (d) Total number of profiles that elicit a Ca2+ response to 1 μM R21A is significantly less than 1 μM TLQP-21 (n = 5 mice/group)