Table 2.

UPLC method for the analysis of Gb₃ isoforms and analogs, and sphingomyelin (C16:0) in PD brain tissue samples.

Column	Acquity UPLC® BEH C8; Waters Corp.
	Lenght: 50 mm
	Internal diameter: 2.1 mm
	Particle diameter: 1.7 μM
Column temperature	22 °C
Weak wash solvent	50% methanol/0.1% formic acid/5 mM ammonium formate/water
Strong wash solvent	0.1% formic acid/methanol
Mobile phase A	0.1% formic acid/5 mM ammonium formate/methanol
Mobile phase B	5% methanol/0.1% formic acid/5 mM ammonium formate/water
Gradient	0–1 min → 50%A
	1–6 min → 50–80%A
	6–12 min → 80–100%A
	15–20 min → 50%A
Flow rate	0.5 mL/min
Injection volume	7.5 μL
Injection mode	Partial loop with needle overfill
Autosampler temperature	22 °C