Table 1.
Results from the various separate repeated-measures ANOVAs are presented for distance traveled, percentage of time spent in perimeter and center entries. Statistics for the main effect of isolation, temporal effects across the testing period, and the isolation × time interactions are shown. Bold font indicates significant results (p ≤ 0.05).
| Isolation | Time | Isolation × Time | |
|---|---|---|---|
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Locomotor Activity – Total Distance traveled by individual fish
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| pfd 10 | F(2,27) = 0.046, p = 0.956 | F(14,378) = 1.398, p = 0.195 | F(28,378) = 1.319, p = 0.183 |
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| 30 | F(2,27) = 2.492, p = 0.102 | F(14,378) = 2.860, p = 0.025 | F(28,378) = 0.802, p = 0.607 |
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| 60 | F(2,42) = 0.169, p = 0.845 | F(14,588) = 0.729, p = 0.565 | F(28,588) = 1.964, p = 0.059 |
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| 90 | F(2,41) = 0.164, p = 0.849 | F(14,574) = 1.287, p = 0.278 | F(28,574) = 0.762, p = 0.634 |
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| 120 | F(2,42) = 1.915, p = 0.160 | F(14,588) = 1.847, p = 0.132 | F(28,588) = 1.988, p = 0.061 |
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| 150 | F(2,41) = 3.194, p = 0.051 | F(14,574) = 1.285, p = 0.283 | F(28,574) = 1.161, p = 0.332 |
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| 180 | F(2,42) = 7.041, p = 0.002 | F(14,588) = 4.198, p = 0.002 | F(28,588) = 1.216, p = 0.285 |
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Anxiety behavior – Time Spent in the Perimeter by individual fish
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| pfd 10 | F(2,27) = 1.192, p = 0.319 | F(14,378) = 2.71, p = 0.010 | F(28,378) = 1.233, p = 0.253 |
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| 30 | F(2,27) = 1.544, p = 0.232 | F(14,378) = 0.632, p = 0.805 | F(28,378) = 0.632, p = 0.805 |
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| 60 | F(2,42) = 1.321, p = 0.278 | F(14,588) = 3.109, p = 0.012 | F(28,588) = 0.782, p = 0.639 |
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| 90 | F(2,41) = 2.768, p = 0.074 | F(14,588) = 2.881, p = 0.019 | F(28,588) = 1.670, p = 0.098 |
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| 120 | F(2,42) = 5.708, p = 0.006 | F(14,588) = 4.639, p < 0.001 | F(28,588) = 1.153, p = 0.327 |
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| 150 | F(2,42) = 2.103, p = 0.135 | F(14,588) = 3.896, p = 0.003 | F(28,588) = 1.030, p = 0.418 |
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| 180 | F(2,42) = 0.805, p = 0.454 | F(14,588) = 5.211, p = 0.001 | F(28,588) = 0.903, p = 0.508 |
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Anxiety behavior – Entries made in the center by individual fish
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| pfd 10 | F(2,27) = 1.267, p = 0.298 | F(14,378) = 2.048, p = 0.038 | F(28,378) = 1.639, p = 0.055 |
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| 30 | F(2,27) = 2.859, p = 0.075 | F(14,378) = 1.231, p = 0.278 | F(28,378) = 1.001, p = 0.454 |
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| 60 | F(2,42) = 1.924, p = 0.159 | F(14,588) = 2.469, p = 0.015 | F(28,588) = 1.312, p = 0.191 |
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| 90 | F(2,41) = 2.422, p = 0.101 | F(14,574) = 2.147, p = 0.047 | F(28,574) = 0.753, p = 0.702 |
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| 120 | F(2,42) = 4.227, p = 0.021 | F(14,588) = 5.087, p < 0.001 | F(28,588) = 1.439, p = 0.141 |
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| 150 | F(2,42) = 1.848, p = 0.170 | F(14,588) = 4.658, p < 0.001 | F(28,588) = 1.307, p = 0.165 |
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| 180 | F(2,42) = 1.655, p = 0.203 | F(14,588) = 4.693, p < 0.001 | F(28,588) = 1.302, p = 0.433 |
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