Table 2.
Recommended extraction solvent and tissue to solvent ratios for best metabolite quantification and lowest variation using the AbsoluteIDQ™ p180 Kit
| Tissue type | Optimal extraction solvent | Optimal tissue to solvent ratio (w/v) |
|---|---|---|
| Liver | EtOH/PB | 1:3 |
| Kidney | MeOH | 1:3 |
| Skeletal muscle (M. quadriceps femoris) | EtOH/PB | 1:3 |
| Fat (visceral) | EtOH/PB | 1:6 |
| Brain (cerebrum) | EtOH/PB | 1:6 |
| Pituitary gland | MeOH | 1:12 |
| Lung | EtOH/PB | 1:3 |
| Bone | EtOH/PB | 1:6 |
| Adrenal gland | EtOH/PB | 1:12 |
| Testis | MeOH | 1:6 |
| Ovary | EtOH/PB | 1:3 |
Tissue to solvent ratios are denoted as 1:X, indicating 1 mg of tissue was homogenized with X µL solvent. Composition of extraction solvents are given in Sect. 2