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. Author manuscript; available in PMC: 2018 Oct 31.
Published in final edited form as: Biochemistry. 2017 Oct 19;56(43):5726–5738. doi: 10.1021/acs.biochem.7b00781

Figure 11.

Figure 11

Co(II)-binding titrations with S100A7ox and S100A7red. Optical absorption spectra titrations (top panels) and Σ563 versus equivalents of Co(II) added (bottom panels) to 300 μM of S100A7ox (A), and S100A7red (B) in 75 mM HEPES, 100 mM NaCl, pH 7.0 and 25 °C. Titrations with S100A7-Ser and S100A7-Ala are presented in Figure S23. Titrations performed in the presence of 2 mM Ca(II) are presented in Figure S24.