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. Author manuscript; available in PMC: 2018 Oct 31.
Published in final edited form as: Biochemistry. 2017 Oct 19;56(43):5726–5738. doi: 10.1021/acs.biochem.7b00781

Figure 7.

Figure 7

Zn(II)-induced response of 2 μM ZP4 in the presence of 5 μM (A) S100A7ox, (B) S100A7red, (C) S100A7-Ser, and (D) S100A7-Ala at pH 7.0 (75 mM HEPES, 100 mM NaCl) at 25 °C, in the absence (black circles) and presence (red circles) of 100 equiv of Ca(II). Excitation was provided at 495 nm and the emission spectra were integrated from 505–650 nm. The integrated emission was normalized to the maximum response (mean ± SDM, n = 3). The data were fit to a two-sites binding model with Kd1 = Kd2. The calculated apparent Kd values corresponding to the fits are listed in the bottom right of each plot.