S100A7 selectively depletes Zn(II) from TSB:Tris medium. Metal analysis of TSB:Tris medium treated with 0–500 μg mL−1 of S100A7ox, S100A7-Ser, and S100A7-Ala. (A) Zn depletion profiles are shown for S100A7ox, S100A7-Ser, and S100A7-Ala. (B) Mn, Fe, Ni, Cu depletion profiles are shown for S100A7ox. The experiments were conducted in the absence (light gray bars) and presence (dark gray bars) of a 2-mM Ca(II) supplement (mean ± SDM, n = 3). The complete metal depletion profiles for S100A7-Ser and S100A7-Ala are presented in Figure S20.