Table 1.
Characterization of S100A7 and Variants
| Protein | HPLC Retention Time (min) a | Free thiol b | Calculated Mass (Da) | Observed Mass (Da) |
|---|---|---|---|---|
| S100A7ox | 16.4 | 0.11 ± 0.03 | 11 454.9 | 11 445.4 11 324.0 (-Met1) |
| S100A7red | 18.4 | 4.16 ± 0.45 | 11 456.9 | 11 457.4 11 326.0 (-Met1) |
| S100A7-Ser | 16.0 | 0.05 ± 0.01 | 11 424.8 | 11 425.5 11 294.2 (-Met1) |
| S100A7-Ala | 16.7 | 0.04 ± 0.00 | 11 392.8 | 11 392.8 11 261.1 (-Met1) |
| S100A7Δox | 21.0 | 0.14 ±0.04 | 11 212.7 | 11 212.9 11 082.0 (-Met1) |
a
The purified proteins are mixtures of full-length S100A7 and S100A7 lacking the Nterminal Met residue. In some chromatograms, partial separation of these two species is observed. The retention time is for the major species, which corresponds to the isoform containing the N-terminal Met residue.
b
Free thiol content determined by using the DTDP assay (mean ± SDM, n = 2).