Skip to main content
. 2017 Dec 11;114(52):13804–13809. doi: 10.1073/pnas.1716097115

Fig. 5.

Fig. 5.

Letrozole infusion into the median eminence suppressed LH release. Blood samples were obtained at 0 h (before), 15, 36, and 48 h after s.c. EB (A and B) or oil (C) injection. In A, letrozole (LZ) was infused 42–48 h after EB, whereas in B, vehicle (V) was similarly infused 42–48 h after EB. Analysis indicated that EB injection induced biphasic changes in LH release (negative and positive feedback effects) (P < 0.0001 for both A and B). LH levels in the EB-injected and letrozole-infused group were also statistically different from EB-injected vehicle infusion (P = 0.01). Oil injection did not induce any LH change. Post hoc analysis indicated that LH levels at 15 h after EB were significantly lower (**P < 0.01 for A, ***P < 0.001 for B) than before EB (0 h). LH levels at 36 h after EB in both EB-treated groups and 48 h in the EB + V group were significantly higher (*P < 0.05 for all) than before EB in respective EB-treated group. Similarly, LH levels at 36 h after EB in EB + LZ and EB + V groups and 48 h after EB + V group were higher than corresponding time in the oil + V group (bP < 0.05; bbP < 0.01). LH levels in the EB-injected letrozole-infused group at 48 h were lower than values at 36 h within the group (#P < 0.05) and at the corresponding time in the EB-injected vehicle-infused group (aaP < 0.01), but values were no longer different from before EB.