Figure 5.

Affinity chemoproteomics revealed BCG_3193 and BCG_3827, novel TB targets with unknown function, as targets of the 6-dialkylaminopyrimidine carboxamides. (A) BCG_3193 and BCG_3827 show reduced binding to 6-dialkylaminopyrimidine carboxamide analogue beads due to compound 40 binding, but no effect on these proteins was seen by the inactive compound. Shown are relative protein amounts captured by the beads in a log 2 scale. (B) Compound structures used for the affinity chemoproteomics experiments. (C) The apparent dissociation constant of compound 40 for BCG_3193 was determined in two different experiments as 0.61 μM and 0.69 μM and for BCG_3827 as 2.1 μM and 3.8 μM.