Figure 3.

Diagram depicting an integrated bioinformatics analysis of exosomes contents and functional reporter assays. Isolated exosomes can be analyzed to profile the genomic, transcriptomic, proteomic, and lipidomic contents. With the generated datasets, multiple integrated analyses can be performed including Gene Ontology (biological process and molecular function) enrichment, protein interaction network, pathway and domain enrichment, and subcellular localization analysis. Administration of biological fluids-derived exosomes or specific cell source-derived exosomes to target cells or animals enables functional characterization. Selective modification of exosome cargo that is predicated upon bioinformatic predictions of effector genes/molecules and delivery to targeted functional reporter assays enables confirmation of mechanistic assumptions.