Fig. 2.

Repression of the hTERT promoter activity by PKC412. a A 3.4-kb hTERT promoter-driven GFP expression was inhibited by PKC412. MV4,11 and MOLM-13 cells were infected with a GFP expression vector driven by a 3.4-kb hTERT promoter and then incubated with or without PKC412. GFP-positive cells were examined and counted under fluorescence microscopy. Right panel: DMSO-treated control cells. Left panel: PKC412-treated cells. b GFP+ cells with and without PKC412 were counted as above and expressed as percentage of total cells. c PKC412-mediated inhibition of the wild-type hTERT promoter activity (p181^wt). MV4,11 and MOLM-13 cells transfected with p181^wt plasmids were treated with PKC412 or DMSO, and luciferase activity was assessed after 24 h (see the “Materials and method” section for details). *P < 0.05; ** P < 0.01. Student’s t test was performed. All the results shown were from three independent experiments