Fig. 5.

hTERT stimulation of the FLT3 downstream effectors and alternative tyrosine kinase (TK) pathways in the presence of PKC412. a Differential expression of c-KIT, DOC3, and SULF2 in MOLM-13-pBMN and MOLM-13-hTERT cells in the presence of PKC412. Cells were treated with PKC412 at 0.1 μM for 24 h, and the mRNA levels of three genes were determined using qPCR. *P < 0.05; ** P < 0.01. Three independent experiments were performed. b The schematic of c-KIT, DOC3, and SULF2 as regulators of the FLT3 and other RTK signaling pathways. c The enhanced Ser473 phosphorylation of AKT in PKC412-treated MOLM-13-hTERT cells. The signal ratios of pAKT/total AKT normalized to Actin were shown. Two independent experiments were performed with similar results