Table 2.
DNA methylation status and age prediction accuracy of single age-related CpG sites measured in GD, EOAD, and GD patients compared to age-matched healthy controls
| Locus CpG site | Disease group | Age group | No. of patients | No. of controls | Mean % of DNA methylation | MAE of predicted and chronological age | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Patients | Controls | P value | Patients | Controls | P value | |||||
| ELOVL2 c7 | GD | Younger | 44 | 39 | 60.34 | 57.59 | 0.055 | 3.918 | 3.861 | 0.935 |
| Older | 47 | 33 | 83.43 | 82.76 | 0.529 | 6.390 | 7.960 | 0.299 | ||
| Total | 91 | 72 | 72.26 | 69.13 | 0.136 | 5.195 | 5.740 | 0.522 | ||
| EOAD | Younger | 17 | 27 | 74.18 | 70.96 | 0.137 | 7.704 | 7.330 | 0.877 | |
| Older | 14 | 30 | 74.36 | 79.33 | 0.253 | 7.285 | 4.522 | 0.100 | ||
| Total | 31 | 57 | 74.26 | 75.37 | 0.566 | 7.530 | 5.852 | 0.237 | ||
| LOAD | Total | 68 | 28 | 83.32 | 84.00 | 0.465 | 11.912 | 9.114 | 0.075 | |
| C1orf132 c1 | GD | Younger | 44 | 39 | 81.89 | 81.33 | 0.633 | 3.704 | 4.935 | 0.159 |
| Older | 47 | 33 | 54.46 | 51.53 | 0.083 | 10.765 | 9.220 | 0.380 | ||
| Total | 91 | 72 | 68.20 | 67.33 | 0.732 | 7.351 | 6.899 | 0.672 | ||
| EOAD | Younger | 17 | 27 | 68.94 | 68.26 | 0.772 | 7.833 | 6.498 | 0.472 | |
| Older | 14 | 30 | 59.00 | 56.20 | 0.279 | 7.384 | 6.640 | 0.652 | ||
| Total | 31 | 57 | 64.45 | 61.91 | 0.236 | 7.631 | 6.573 | 0.387 | ||
| LOAD | Total | 68 | 28 | 48.44 | 50.61 | 0.281 | 11.326 | 11.273 | 0.967 | |
| FHL2 c2 | GD | Younger | 44 | 39 | 33.61 | 36.56 | 0.028 | 3.793 | 8.026 | 0.001 |
| Older | 47 | 33 | 56.98 | 57.70 | 0.586 | 8.031 | 8.023 | 0.994 | ||
| Total | 91 | 72 | 45.68 | 46.25 | 0.776 | 5.982 | 8.025 | 0.012 | ||
| EOAD | Younger | 17 | 27 | 41.59 | 41.30 | 0.900 | 8.836 | 8.012 | 0.709 | |
| Older | 14 | 30 | 48.57 | 51.93 | 0.119 | 9.047 | 5.539 | 0.215 | ||
| Total | 31 | 57 | 44.74 | 46.89 | 0.256 | 8.931 | 6.711 | 0.198 | ||
| LOAD | Total | 68 | 28 | 60.60 | 60.43 | 0.889 | 10.243 | 8.357 | 0.060 | |
| TRIM59 c7 | GD | Younger | 44 | 39 | 30.43 | 26.24 | 0.0001 | 8.609 | 4.944 | 0.003 |
| Older | 47 | 33 | 49.60 | 49.94 | 0.866 | 9.663 | 9.734 | 0.965 | ||
| Total | 91 | 72 | 40.33 | 37.25 | 0.132 | 9.153 | 7.170 | 0.056 | ||
| EOAD | Younger | 17 | 27 | 40.76 | 35.00 | 0.004 | 12.176 | 5.653 | 0.008 | |
| Older | 14 | 30 | 48.93 | 44.23 | 0.013 | 6.805 | 7.264 | 0.803 | ||
| Total | 31 | 57 | 44.45 | 39.86 | 0.006 | 9.751 | 6.500 | 0.023 | ||
| LOAD | Total | 68 | 28 | 54.32 | 52.86 | 0.385 | 9.970 | 10.033 | 0.955 | |
| KLF14 c1 | GD | Younger | 44 | 39 | 4.20 | 4.56 | 0.261 | 5.082 | 8.873 | 0.002 |
| Older | 47 | 33 | 10.11 | 11.18 | 0.083 | 12.101 | 10.751 | 0.414 | ||
| Total | 91 | 72 | 7.25 | 7.60 | 0.564 | 8.707 | 9.734 | 0.344 | ||
| EOAD | Younger | 17 | 27 | 10.18 | 6.74 | 0.013 | 14.478 | 9.076 | 0.096 | |
| Older | 14 | 30 | 8.07 | 9.43 | 0.206 | 14.366 | 9.714 | 0.035 | ||
| Total | 31 | 57 | 9.23 | 8.16 | 0.239 | 14.427 | 9.415 | 0.016 | ||
| LOAD | Total | 68 | 28 | 13.24 | 11.89 | 0.102 | 13.247 | 12.234 | 0.653 | |
Significant values marked with italics