FIG 2.

The enzyme activity of USP6 is important for its ability to regulate the stability of the c-Jun protein. (A) An empty vector or a plasmid expressing USP6 or USP6 C541S was transfected into HeLa cells, and the cells were harvested 36 h later. Whole-cell lysates were subjected to SDS-PAGE and analyzed by immunoblotting with the indicated antibodies. (B) The same assay as in panel A, except that the plasmids were transfected into MCF7 cells. (C) The same assay as in panel A, except that the empty vector or USP6-expressing plasmid was transfected into HeLa cells. (D) The indicated plasmids were transfected into MCF7 cells, and the cells were harvested 24 h later. Whole-cell lysates were subjected to SDS-PAGE and analyzed by immunoblotting with the indicated antibodies. All experiments were performed at least 3 times.